Yet, ALPK1, a cytosolic immune receptor identified in Yersinia pseudotuberculosis and S. flexneri infection in epithelial cell lines (33, 35), was not a genetic hit in our genome-wide CRISPR screens, possibly because this receptor has different functions in epithelial cells and macrophages during intracellular pathogen infection (Fig. 2B). This evidence concerns the gene ALPK1 and infection.