GSDMD and infection: In contrast, the survival of cells with knockout or knockdown of AIM2, NLRP3, CASP1, or GSDMD was slightly shortened in S. flexneri ΔvirG infection, indicating that these genes in the pyroptotic cell death pathway actually may not be detrimental to host cells infected with S. flexneri (Fig. 2B and C; see also Table S5 at https://doi.org/10.17632/xn3vv2cdnk.1).