The aims of this study were to explore the function of miR-541-5p in HCC progression and to study whether circMTO1 functions as a suppressor of HCC progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling and the EMT process, which may provide a new research direction for targeted HCC therapy. The gene discussed is ZIC1; the disease is hepatocellular carcinoma.