In the presence of doxycycline, we observed a robust induction of PUMA at both the mRNA and protein levels in KG1a and MOLM-13 cells transfected with WTIP vector (Fig. 3D, E), suggesting that PUMA is involved in WTIP overexpression induced apoptosis in AML cells. The gene discussed is BBC3; the disease is acute myeloid leukemia.