LRG1 and colorectal carcinoma: To validate RNA sequencing data, 60 unpaired primary (n = 30) and liver metastatic CRC (n = 30) in addition to 6 paired primary and liver metastatic CRC samples were subjected to quantitative reverse transcription PCR (qRT-PCR) analysis, and the data confirmed a significant increase of LRG1 in the LM lesions (Fig. 1b).