In order to study the effect of Nef on the development of HSPCs into myeloid and erythroid lineage cells and in consideration of the low efficiency of direct infection of HSPCs with HIV-1 in vitro, in current study we delivered nef via lentiviral (LVX-EF1α-LAINef-IRES-zsgreen1, LVX-nef) transduction into CD34 + HSPCs enriched from human cord blood. This evidence concerns the gene S100B and infection.