EA decreased the expression of P2Y12 and IL-1β as well as the activation of microglia, suggesting that EA downregulated the expression of P2Y12, weakened the activation of microglia, and then inhibited IL-1β expression in the mPFC, thus relieving hyperalgesia and depression in IBD mice (Fig. 10). The gene discussed is P2RY12; the disease is irritable bowel syndrome.