In addition, given the lack of genetic characteristics and heterogeneity of tumors derived from xenograft mouse model assay, the in vivo data should be confirmed over a longer period of time using patient-derived tumor xenograft (PDX) model, in vivo assay only testified the role of Rg3 in tumor growth, and further experiments should be designed to confirm the effects of circ_0003074/miR-516b-5p/KPNA4 pathway on Rg3-mediated OS development in vivo. Here, KPNA4 is linked to neoplasm.