SIRT2 and infection: Interaction of SIRT2 with TDP-43 upon infection was determined by cell fractionation and immunoprecipitation, HeLa cells were transfected with plasmids encoding GFP alone or GFP-tagged SIRT2 (SIRT2-GFP), then left uninfected or infected with L. monocytogenes followed by immunoprecipitation from isolated nuclei.