Furthermore, since MGMT and IDH1/2 have been established as the biomarkers for glioma treatment, we also detected the effects of OTUB1 on the protein expression of MGMT and IDH1/2 in glioma cells and found that OTUB1 knockdown decreased MGMT protein expression, while failed to affect IDH1/2 protein expression (Supplementary figure S1); however, the concrete mechanisms underlying OTUB1-mediated regulation on MGMT expression should be explored in the future. This evidence concerns the gene IDH1 and central nervous system cancer.