We assessed the quantity of NF-κB p65 protein from drug-treated ESCC cell spheres using Western blot analysis, to evaluate whether blockage of NK1R could increase the cytotoxicity of aprepitant through suppression of NF-κB. Furthermore, the RT-PCR study of antiapoptotic NF-κB target genes has examined the impact of aprepitant on the NF-κB axis. This evidence concerns the gene TACR1 and esophageal squamous cell carcinoma.