CD4 and melanoma: FoxP3+ frequencies within the CD4+ T cell subset in fresh PBMC for this patient cohort (n = 11) were higher compared to fresh PBMC isolated from healthy controls (n = 10; C14-23) (p = 0.043) (Figure 7A; Supplementary Figure 7B), although the difference was modest as shown for other melanoma patients (Figure 3B).