Single-factor and LASSO Cox regression analysis of abnormally expressed RBPs, resulted in 10 key prognostic RBPs (TDRD6, TLR8, NXT2, EIF4E3, RPS27L, CPEB3, RBM34, TERT, RPS29, and ZC3HAV1), and an RBP prognostic risk assessment model for patients with osteosarcoma was successfully constructed. Here, TDRD6 is linked to osteosarcoma.