Seven differentially expressed proteins were identified: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE1), thrombospondin-1 (THBS1), pro-platelet basic protein (PPBP), secreted phosphoprotein-2 (SPP2), insulin-like growth factor-2 (IGF2), cadherin 1 (CDH1), and apolipoprotein C 4-2 (APOC4-APOC2), four of which were elevated (LYVE1, THBS1, PPBP, and IGF2) and subsequently validated by Western blot analysis in patients with AIS caused by LVO versus healthy control subjects. The gene discussed is SPP2; the disease is androgen insensitivity syndrome.