For example, BRCA1, which did not change significantly between breast cancer and non-cancer samples in the raw data (p = 0.461, Wilcoxon rank-sum test), showed significantly lower TSS coverage in breast cancer patients after imputation (p = 0.0480, Wilcoxon rank-sum test), which is consistent with the high expression level of BRCA1 in breast cancer tissues (Figure 4E). This evidence concerns the gene BRCA1 and breast cancer.