In addition to the effects on Fgf10, our data show that transpulmonary pressure affects the expression of several other genes associated with lung development, including Spry2, Sftpc, and Cftr. Consistent with our results, a recent study showed that Sftpc is a direct downstream target of FGF10 signaling in the pseudoglandular-stage murine lung (Isago et al., 2020), and Sftpc expression is also reduced in the nitrofen rat model of CDH (Guilbert et al., 2000). This evidence concerns the gene SPRY2 and congenital diaphragmatic hernia.