Biological studies related to renal fibrosis showed that (1) 11 inhibits over-expression of fibronectin, collagen I and α-SMA, (2) 4, 6 and 8 significantly inhibitof the migration ability of BT549 cells, (3) 6 decreases the protein level of TWIST1 and ZEB1 and increases the protein level of E-cadherin, and (4) 7, 9, 11 and 15 significantly up-regulate p-AMPK protein expression in normal L6 myotubes cells. The gene discussed is TWIST1; the disease is renal fibrosis.