In our experiments, the levels of TJ proteins (Claudin-5, JAM1), GJ protein (CX43), and AJ protein (VE-cadherin) decreased, while the levels of IL-1β and TNF-α increased, suggesting that the mucosal barrier function in colitis induced by TNBS was destroyed, accompanied with high expression of inflammatory cytokines. This evidence concerns the gene CLDN5 and colitis.