Additionally, when a mutant PICV virus (rPICV-NPmut) that is known to be unable to suppress IFN-I expression was used to infect the WT as well as the SKO and DKO mice, all mice were able to effectively control virus infection, which further emphasizing the important roles of innate and adaptive immune responses that include but are not necessarily limited to RIG-I and MDA5, in controlling PICV infection in mice. This evidence concerns the gene IFIH1 and viral infectious disease.