CD8A and neoplasm: To determine the phenotype of these five clusters (Clusters 0, 2, 5, 4, and 8) (Figure 3A), we compared the gene signatures of each intratumoral CD8+ T cell population against signatures of CD8+ T cell activation and exhaustion obtained from public datasets (30) using gene set variation analysis (GVSA) (Figure 3B) and queried genes characteristic of tumor-infiltrating T cell phenotypes (Figure S4D).