To seek compounds effective in treating the most malignant type of GBM with EGFR amplification and PTEN deletion, we developed a cell-based screening assay to identify individual compounds from Traditional Chinese Medicine that specifically block the cell proliferation of U87MG-EGFR with EGFR amplification and PTEN deficiency but mildly inhibit the proliferation of U87MG-PTEN cells without EGFR amplification and with wild-type PTEN. This evidence concerns the gene EGFR and glioblastoma.