We found a significant decrease in the abundance of known homeostatic microglial markers, P2RY12 and TMEM119, and a corresponding increase in DAM markers, FTH1 (Stage 1 DAM) and TREM2 (Stages 1 and 2 DAM), in microglial EVs from the AD brain when compared to NL. This evidence concerns the gene TREM2 and Alzheimer disease.