Bufalin increased the expression of necrosis mediators threonine kinase1 and threonine kinase3, induced MDA-MB-231 cell death and inhibited the growth of both human breast cancer MCF-7 and MDA-MB-231 cells through the reactive oxygen species-mediated RIP1/RIP3/PARP-1 pathway (Li et al., 2018b). Here, PARP1 is linked to breast cancer.