ACLY and cancer: Cancer cells expressed a splice isoform of the glycolytic enzyme pyruvate kinase (M2), which was necessary for tumor formation in nude mouse xenografts.310 During Akt-mediated tumorigenesis, the conversion of glucose to lipid depends on the activity of ATP citrate lyase (ACL), which promotes the production of cytosolic acetyl-CoA from mitochondria-derived citrate.311 Conversely, mutations in some oncogenes can affect cancer-associated metabolic reprogramming.