At multivariate analysis, older than 45 years (HR 7.194, 95% CI: 3.259–15.884; P < 0.001), ALT > 50 U/l (HR 20.488, 95% CI: 1.012–60.190; P = 0.046), AST > 40 U/l (HR 27.323, 95% CI: 1.478–55.122; P = 0.026), PLT < 100(× 109/l) (HR 61.246, 95% CI: 7.361–109.604; P < 0.001) and detectable HIV-RNA (HR 27.850, 95% CI: 1.607–81.401; P < 0.001) were risk factors for liver fibrosis in individuals with HIV/HBV co-infection. The gene discussed is GPT; the disease is coinfection.