PTEN and osteosarcoma: Furthermore, we explored and confirmed the molecular mechanisms of its inhibitory effects on osteosarcoma growth mainly by RNA-seq, qRT-PCR and Western blot assays, which is, induction of autophagy and G0/G1 phase arrest by inactivating the PTEN-PI3K-AKT-mTOR/p21 signaling pathway via targeting and up-regulating the expression of PTEN.