As shown in Fig. 6A and B, diosmetin markedly inhibited the proliferation ability of osteosarcoma cells, while IL-6 administration, which could simulate STAT3 signaling activation, counteracted diosmetin-induced anti-osteosarcoma effect and significantly, although partly, reverse proliferation of Saos-2 or U2OS cells. The gene discussed is IL6; the disease is osteosarcoma.