STAT3 and osteosarcoma: To further verify the dependent role of STAT3 signaling in the cellular response elicited by diosmetin, osteosarcoma cells transfected with HA-STAT3 and Flag-STAT3 expression plasmid were either left uninduced or stimulated with 30 μM diosmetin for 24 h, and STAT3 was immunoprecipitated from cell lysates with a Flag-STAT3 antibody, separated by SDS–PAGE, analyzed by western blot with a HA-STAT3 antibody.