The expression of p-AKT, AKT and PFKFB3 in tumor tissues was also detected at the animal level, and we found that the expression of p-AKT and PFKFB3 was significantly decreased in the shRNA-TKTL1 group compared with the control group (Fig. 9E), which was consistent with the results of cell experiments. The gene discussed is TKTL1; the disease is neoplasm.