The fifth cohort of mice was transplanted with bone marrow cells and 1 × 106 CD8+ T from WT C57Bl/6, challenged with luciferase expressing B‐ALL cells, and further treated with 0.5 × 106 canonical Tregs sorted from ITK‐deficient mice by CD25+ FOXP3RFP. The gene discussed is ITK; the disease is acute lymphoblastic leukemia.