As shown in Figure 8 (shRNA lentiviral knockdown) and Figure 8—figure supplement 1 (siRNA knockdown), cleaved caspase-3 expression level in p53 and NMNAT double knockdown cells was reduced compared to those in siNMNAT cells, suggesting that p53 depletion reduced significantly cleaved caspase-3 expression in NMNAT knockdown glioma cells. Here, CASP3 is linked to glioma.