We used Repo and DAPI to label the nuclei region and observed a significant decrease of caspase-3 levels in glial neoplasms that overexpress Nmnat-PC (nuclear), compared with those overexpressing lacZ, Nmnat-PCWR (nuclear), or Nmnat-PD (cytoplasmic) (Figure 4A and C). The gene discussed is CASP3; the disease is glioma.