Additionally, transcription levels of genes coding for the NLRP3 inflammasome components NLRP3 and PYCARD, downstream signalling adaptors (NFκBIA and CARD9), enzymes (CASP10, AZI2, PACT and TBK1) and the IFNLR1 receptor fluctuated around basal values independently of the animals, days of sample collection and the strain used for infection (Supplementary Figure 3(a) and Supplementary Data S1). This evidence concerns the gene PYCARD and infection.