For instance, SNU‐4849 harbored multiple tumor driver mutations, such as APC (c.4132C > T/p.Q1378*), TP53 (c.713G > A/p.C238Y), and ARID1A (c.4187_4188del/p.G1396Afs*48), with an allele frequency of ≈0.95 in the trunk, while each of the subregional clones shaped the phylogenetic tree with individual mutations of ≈0.2 VAFs (Figure 2B; Table S2B, Supporting Information). This evidence concerns the gene APC and neoplasm.