The AUC cutoff values of circulating miRNAs, miR-146a and miR-144; myokines, adropin and irisin; and serum OPN were significantly identified by ROC analysis which additionally recommends the potential use of these biomarkers: miR-146a, miR-144, adropin, irisin, and OPN as diagnostic biomarkers with considerable specificity and sensitivity for diabetes in patients with varying glycemic control status. This evidence concerns the gene SPP1 and diabetes mellitus.