ALB and infection: To address the lack of proteins in MHB and better mimic in vivo conditions at the infection site, two main approaches are commonly described in the literature: (i) Incorporate serum to the test medium, ensuring bacterial growth is not inhibited by the adjusted medium since serum contains antimicrobial-acting substances (34), or (ii) Incorporate albumin alone to the test medium as this protein represents the major actor in drug binding (29, 34).