We further stratified the entire TCGA-LIHC cohort into different subgroups according to the clinical characteristics including age (≥65 or <65 years), sex (male or female), AJCC stage (stage I-II or stage III-IV), pathological T (T1-2 or T3-4), pathological M (M0 or M + ), pathological N (N0 or N+), tumor histopathological grade (G1-2 or G3-4), AFP level (high ≥400 ng/ml or low <400 ng/ ml). The gene discussed is AFP; the disease is neoplasm.