Above all, we cannot completely deny the possibility that Nrf2 directly orchestrates the transcription of Ccl2 and Ccl7; the reasons are as follows: the samples for CHIP-seq analysis were not samples of mice treated with myocardial ischemia-reperfusion for 6h; CHIP-seq analysis dependent on anti-Nrf2 antibody does not necessarily reflect true and accurate results in the samples of mice treated with IR6h. Here, STUB1 is linked to myocardial ischemia.