NTG and reserpine can regulate vascular tension and neurotransmitter balance by regulating vasomotor factors such as NO, CGRP, and ET-1 and neurotransmitters such as 5-HT, resulting in the activation and accumulation of proinflammatory mediators, finally leading to migraine (Sun et al., 2017; Wang et al., 2019). The gene discussed is EDN1; the disease is migraine disorder.