BRAF and RASopathy: As expected, triggering BRAF(V600E) expression at a later stage of zebrafish embryonic development was sufficient to induce visible malignancies such as craniofacial deformities, cardiac malformation, and cutaneous abnormalities, all of which were reminiscent of cardio-facio-cutaneous (CFC) syndrome, a RASopathy commonly caused by BRAF mutations33–36 (Fig. 3i–p and Supplementary Fig. 8a–j).