To test whether DDX41 also opposes DSBs and genomic instability in hematopoietic stem and progenitor cells (HSPCs) in which loss-of-function mutations of DDX41 result in AML, we depleted DDX41 in human CD34+ HSPCs, and monitored DSBs using 53BP1 foci. Here, DDX41 is linked to acute myeloid leukemia.