To further clarify the effect of mTORC1 signaling, we treated HMGB1/RICTOR 3′UTR overexpressed HCC cells (OE-HMGB1/RICTOR groups) with Rapamycin (a mTORC1 specific inhibitor) and estimated the PD-L1 levels. The gene discussed is HMGB1; the disease is hepatocellular carcinoma.