To study whether Tlr4 played important roles in colorectal tumorigenesis in miR-148a–/– mice, we crossed Tlr4–/– mice with miR-148a–/– mice to generate miR-148a–/– Tlr4–/– mice and analyzed CRC development following AOM/DSS treatment. The gene discussed is TLR4; the disease is colorectal carcinoma.