Consistent with previous results, we further validated that ANXA1 was mainly upregulated in MES gliomas and macrophages, especially overexpressed in the pseudopalisading cells around the necrosis and microvascular proliferation region which further precisely confirmed the location of ANXA1, indicating that ANXA1 could drive transitions to MES-like states in gliomas and plays an important role in M2 macrophages to induce the inhibitory glioma microenvironment. The gene discussed is ANXA1; the disease is central nervous system cancer.