Furthermore, they used three different mouse models to investigate PTPMT1 in vivo: a hydrodynamic tail vein injection (HDTVi) model, where they injected a p53 KO by CRISPR-Cas9-KO plasmid and c-myc oe by sleeping beauty (SB) transposon system plasmid in C57BL/6N mice; a subcutaneous tumor model with nude mice using MHCC97L cells and orthotopic tumors with luciferase-labeled MHCC97L cells in nude mice. Here, TP53 is linked to neoplasm.