In line with previous reports (37), we observed low basal levels of EGFR phosphorylation (pEGFR, Tyr1068) in the TKCC-OSCC-22 tumor culture, whereas pEGFR protein levels were 55% higher in the TKCC-OSCC-16 model, suggesting activation of this signaling axis in the EGFR-amplified line (Figure 6C). The gene discussed is EGFR; the disease is neoplasm.