TFS inhibited clonal expansion, and tumor sphere formation reduced the cells' self-renewal capacity and was shown to downregulate the expression of p-AKT, p-GSK-3β, β-catenin, and c-Myc proteins while upregulating the phosphorylation of β-catenin thereby inhibiting the Wnt/β-catenin signaling pathway. This evidence concerns the gene GSK3B and neoplasm.