To further characterize the systemic immune activation after challenge and re-challenge of mice with tumor cells, the total immune cells in the draining lymph nodes were counted (Supplementary Figure S1F), and phenotypic analysis of CD4 (naive, CM, EM), CD8 (naive, CM, EM), and NK and NKT cell populations was performed (Supplementary Figure S1G). Here, CD4 is linked to neoplasm.