CD40L:CD28-transduced TCR-T58 T cells were co-cultured with the melanoma tumor cell line (SK-Mel23) (Figure 6A), which provides the activation stimulus through the TCR-peptide/MHC interaction (HLA-A2/tyrosinase, which is the ligand for TCR-T58) and expresses CD40 endogenously. This evidence concerns the gene TYR and melanoma.