The low expression of neuronal markers in SOD1 G93A-Tg ALS mouse was remarkably alleviated by PRG-A01 treatment compared to no treatment (MAP2: 125.1 % improvement, p < 0.0011 and NeuN: 86.4% improvement, p < 0.035) (Fig. 4h, i and S9a–e) and the pathological inclusions of SOD1 in neuronal cells was ameliorated by reduction of 35.5 % (p < 0.014) (Fig. 4f and S7). The gene discussed is MAP2; the disease is amyotrophic lateral sclerosis.