Our chemical, PRG-A01, showed the protective effects on muscle weakness (49.1 % improvement, p < 0.0121) and movement disability (velocity 60.8% and distance 66.2% improvement, p < 0.0188, p < 0.00088, respectively) shown in SOD1 G93A-Tg ALS model mouse (Fig. 4c–e and S5c–f), and histological analysis revealed that motor neuron viability in spinal cord was improved by 86 % (p < 0.00015) in PRG-A01 treated mice (Fig. S8a–d). This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.