When we analyzed the trans-regulatory differences between prostate tumours with a secondary Gleason pattern 5 (Gleason score 4 + 5) to tumours with Gleason pattern 4 (Gleason score 4 + 4) we find an enrichment of binding sites for class I steroid receptors, androgen (ARE), glucocorticoid (GRE) and progesterone (PG) (Fig. 4c). The gene discussed is JUP; the disease is prostate neoplasm.