Interestingly, Sample_15, a Gleason pattern 4 tumour, shows high enrichment for FOXA1, HOXB13 and CDX2, followed by Fra1/2, Atf3, JunB and AP-1 binding sites enriched in Gleason pattern 3 tumours, suggesting a transitionary state between the two regulatory states (Fig. 4b). This evidence concerns the gene JUN and neoplasm.