Altogether, our data suggested that MTHFD1L might have a significant contribution to the development and progression of BLCA, HNSC, KIRP, LUAD, and UCEC, thus the next part of our study will mainly focus on the unique role of MTHFD1L in these five types of human cancers. The gene discussed is MTHFD1L; the disease is bladder transitional cell carcinoma.