Consistent with the above findings, measurement of cardiac hypertrophy marker levels using quantitative reverse transcription-polymerase chain reaction(RT-qPCR) revealed a persistent increase in myosin heavy chain 7(Myh7) levels by PO-2d and a significant increase in natriuretic peptide a (Nppa), actin alpha 1 (Acta1), and natriuretic peptide b (Nppb) levels by PO-7d, while a decrease in Phospholamban (PLN) levels from PO-7d, indicating the onset of decompensated cardiac hypertrophy (Fig. 1L). This evidence concerns the gene ACTA1 and cardiac hypertrophy.